ABSTRACT
OBJECTIVE: To discuss the effect of feiliuping ointment on proliferation, angiogenesis and PI3K/AKT pathway of rat bone marrow-derived endothelial progenitor cells (EPCs) in A549 lung tumor cells supernatant. METHODS: Bone marrow mononuclear cells were isolated from Sprague-Dawley rats by density gradient centrifugation methods and cultured in EGM-2 MV medium. And fluorescent immunocytochemistry was used to detect CD133 and vascular endothelial growth factor receptor 2 (VEGFR2) expression. EPCs were treated with 0.9% sodium chloride solution (blank control) or serum of rats treated with high dose and low dose Feiliuping ointment for 48 h to explore the concentration-effect, or with serum of rats treated with high dose feiliuping ointment for 0, 12, 24, 48 h to explore the time-effect. MTT assay and Matrigel method were used to detect the proliferation and angiogenesis of EPCs. The NF-κB, p-Akt, PI3K p85 proteins of EPCs were detected by Western blot. RESULTS: After induced culture for 7 d, the isolated bone marrow mononuclear cells exhibited round or short spindle-shaped appearance, were Dil-Ac-LDL and FITC-UEA-1 positive, and expressed CD133+VEGFR2+. After being treated with high dose feiliuping ointment serum for 48 h, OD490 nm value was lower than that in the control group or low dose feiliuping ointment group (P<0.05). The inhibition effect of rat serum after Feiliuping ointment administration on EPCs was dose-dependent and time-dependent. Matrigel tube formation assays showed that the counts of tube formation of EPCs in high dose Feiliuping ointment group was lower than that in control group or low dose of feiliuping ointment group (P<0.05). And the levels of NF-κB, p-Akt, PI3K p85 proteins in high dose of feiliuping ointment group were lower than those in control group(P<0.01). CONCLUSION:S Treatment with administration can Feiliuping ointment serum would inhibit the proliferation and tube formation of bone marrow-derived EPCs in A549 supernatant via the down-regulation of PI3K/AKT signaling pathway.
ABSTRACT
Objective To study the effect of Feiliuping ointment combined with chemotherapy on the cellular immune function and related inflammatory factors in patients with lung cancer. Methods A total of 202 patients with non-small cell lung cancer (NSCLC) admitted to Henan Cancer Hospital from May 2014 to December 2016 were selected as subjects. They were randomly divided into the simple chemotherapy group (control group) and Feiliuping Ointment combined with chemotherapy group (observation group), and there were 101 cases in each group. The short-term curative effect, cellular immune function, and levels of inflammatory factors before and after treatment were observed and the adverse reactions were recorded. Results The effective rate and stability rate in the observation group were significantly higher than those in the control group (P < 0.05). Levels of CD3+, CD4+, CD4+/CD8+, and NK in the observation group after treatment were significantly higher than those in the control group (P < 0.05), while CD8+ was significantly lower than the control group (P < 0.05). Compared with the control group, the levels of serum IL-6, TNF-α, and hs-CPR were significantly lower in the observation group after treatment (P < 0.05). The incidence of adverse reactions in the observation group after treatment was significantly lower than that in the control group (P < 0.05). Conclusion Feiliuping Ointment combined with chemotherapy can effectively improve the clinical curative effect on lung cancer, regulate the immune mechanism, reduce the levels of inflammatory factors, improve the condition, and reduce the occurrence of adverse reactions in patients, thus deserving promoting.